Investigating Ochratoxin-A Biodegradation, Detoxification, and Antibiotic Resistance Ability of Brevundimonas naejangsanensis
DOI:
https://doi.org/10.55544/sjmars.3.4.11Keywords:
OTA, biodegradation, detoxification, antibiotic, resistanceAbstract
Ochratoxin A (OTA) is a nephrotoxic and genotoxic mycotoxin produced by Aspergillus and Penicillium species. It threatens animal and human health by contaminating feed and food commodities. Biodegradation is the most widely utilized method for detoxifying this mycotoxin. Brevundimonas naejangsanensis was reported to biodegrade 60 % of OTA at 4 mg/l during the first day, 85% on the second day, and almost 99-100 % at the end of the third day when using 10 ppm of OTA. The capacity of B. naejangsanensis was examined to break down OTA at 28 and 30 °C in LB medium at concentrations of 4 ppm and 10 ppm. Under these circumstances, B. naejangsanensis was able to degrade almost 100% of OTA starting at a concentration of 10 (g/ml). B. naejangsanensis eliminated an average of 0.1008 and 0.05455 (g/ml/h) of OTA from a medium having an initial concentration of 4 (g/ml) at 28 and 30 °C, respectively. At both incubation temperatures, Ochratoxin A was halted significantly by B. naejangsanensis at both log phase and after finishing the log phase of cell development. According to the results, OTA was decomposed by B. naejangsanensis to ochratoxin of lower toxicity. At the same time a strain of microorganism was being tested for its antimicrobial potential, with tests demonstrating that such resistance might be achieved. The antibiotic susceptibility of a B. naejangsanensis strain isolated from soil samples was evaluated against a range of unrelated antibiotic drug classes. A. calcoaceticus was resistant to most of the antibiotics. Through the use of several methods, including high-performance liquid chromatography (HPLC), the toxin's breakdown was tracked.
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